Nuclear Microsatellite Primers for the Endangered Relict Fir, Abies pinsapo (Pinaceae) and Cross-Amplification in Related Mediterranean Species

International Journal of Molecular Sciences

Jose M. Sánchez-Robles¹  Francisco Balao¹  Juan L. Garc໚-Castaño¹  Anass Terrab¹  Laura Navarro-Sampedro² 

[1] Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Apdo. 1095, Sevilla E-41080, Spain; E-Mails:;Centro de Investigación, Tecnología e Innovación (CITIUS), Universidad de Sevilla, Apdo. 1095, Sevilla E-41080, Spain; E-Mail:

关键词: Abies;    genetic conservation;    mediterranean basin;    nSSR;    Pinaceae;    population genetics;   

DOI  :  10.3390/ijms131114243

来源: mdpi

【 摘 要 】

Twelve nuclear microsatellite primers (nSSR) were developed for the endangered species Abies pinsapo Boiss. to enable the study of gene flow and genetic structure in the remaining distribution areas. Microsatellite primers were developed using next-generation sequencing (454) data from a single Abies pinsapo individual. Primers were applied to thirty individuals from the three extant localities. The number of alleles per locus ranged from one to four. Cross-amplification was tested for other Abies species from the Mediterranean Basin, and most of the loci showed higher polymorphisms in the Mediterranean species than in A. pinsapo. These microsatellite markers provide tools for conservation genetic studies in Abies pinsapo as well other Abies species from the Mediterranean Basin.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.