Structural Probes in Quadruplex Nucleic Acid Structure Determination by NMR

Molecules

Andreas Ioannis Karsisiotis¹ 

[1] id="af1-molecules-17-13073">Biomedical Sciences Research Institute, University of Ulster, Cromore Road, Coleraine, Co. Londonderry BT52 1SA,

关键词: chemical modifications;    8-bromoguanosine;    quadruplex;    2;    6-diaminopurine;    LNA;    inosine;    8-O-methylguanine;    8-methylguanine;    5-bromouracil;    riboguanosine;   

DOI  :  10.3390/molecules171113073

来源: mdpi

【 摘 要 】

Traditionally, isotope-labelled DNA and RNA have been fundamental to nucleic acid structural studies by NMR. Four-stranded nucleic acid architectures studies increasingly benefit from a plethora of nucleotide conjugates for resonance assignments, the identification of hydrogen bond alignments, and improving the population of preferred species within equilibria. In this paper, we review their use for these purposes. Most importantly we identify reasons for the failure of some modifications to result in quadruplex formation.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.