Immuno-MALDI-MS in Human Plasma and On-Chip Biomarker Characterizations at the Femtomole Level

Sensors

Alain Rouleau²  Marven El Osta¹  Géraldine Lucchi¹  Patrick Ducoroy¹ 

[1] SFR Santé STIC, CLIPP (Clinical-Innovation Proteomic Platform), Université de Bourgogne, Centre Hospitalier Universitaire de Dijon, 21000 Dijon, France; E-Mails:;Institut FEMTO-ST, Université de Franche Comté, CLIPP (Clinical-Innovation Proteomic Platform), 25044 Besançon, France; E-Mail:

关键词: Surface Plasmon Resonance;    mass spectrometry;    immuno MALDI-MS;    biomarker;    proteomics;   

DOI  :  10.3390/s121115119

来源: mdpi

【 摘 要 】

Immuno-SPR-MS is the combination of immuno-sensors in biochip format with mass spectrometry. This association of instrumentation allows the detection and the quantification of proteins of interest by SPR and their molecular characterization by additional MS analysis. However, two major bottlenecks must be overcome for a wide diffusion of the SPR-MS analytical platform: (i) To warrant all the potentialities of MS, an enzymatic digestion step must be developed taking into account the spot formats on the biochip and (ii) the biological relevancy of such an analytical solution requires that biosensing must be performed in complex media. In this study, we developed a procedure for the detection and the characterization at ∼1 μg/mL of the LAG3 protein spiked in human plasma. The analytical performances of this new method was established, particularly its specificity (S/N > 9) and sensitivity (100% of LAG3 identification with high significant mascot score >68 at the femtomole level). The collective and automated on-chip MALDI-MS imaging and analysis based on peptidic fragments opens numerous applications in the fields of proteomics and diagnosis.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.