Dose and Latency Effects of Leucine Supplementation in Modulating Glucose Homeostasis: Opposite Effects in Healthy and Glucocorticoid-Induced Insulin-Resistance States

Nutrients

Nelo Eidy Zanchi⁵  Lucas Guimar฾s-Ferreira³  Mário Alves de Siqueira-Filho³  Vitor Felitti⁵  Humberto Nicastro⁵  Carlos Bueno¹  Fปio Santos Lira⁴  Marshall Alan Naimo²  Patrໜia Campos-Ferraz⁵  Maria Tereza Nunes³  Marília Seelaender⁴  Carla Roberta de Oliveira Carvalho³  François Blachier⁶ 

[1] Human Genome Research Center, Institute of Biosciences, University of São Paulo, São Paulo, 05508-900, Brazil;Department of Health Sciences and Human Performance, The University of Tampa, Tampa, FL 33606, USA;Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, 05508-900, Brazil;Cancer Metabolism Research Group, Institute of Biomedical Sciences, University of São Paulo, Sao Paulo, 05508-900, Brazil;Laboratory of Applied Nutrition and Metabolism, School of Physical Education and Sports, University of Sao Paulo, Sao Paulo, 05508-030, Brazil;INRA, CRNH-IdF, UMR 914, Nutrition Physiology and Ingestive Behavior, Paris, 78350, France;

关键词: leucine supplementation;    glucose homeostasis;    skeletal muscle mass;   

DOI  :  10.3390/nu4121851

来源: mdpi

【 摘 要 】

Dexamethasone (DEXA) is a potent immunosupressant and anti-inflammatory agent whose main side effects are muscle atrophy and insulin resistance in skeletal muscles. In this context, leucine supplementation may represent a way to limit the DEXA side effects. In this study, we have investigated the effects of a low and a high dose of leucine supplementation (via a bolus) on glucose homeostasis, muscle mass and muscle strength in energy-restricted and DEXA-treated rats. Since the leucine response may also be linked to the administration of this amino acid, we performed a second set of experiments with leucine given in bolus (via gavage) versus leucine given via drinking water. Leucine supplementation was found to produce positive effects (e.g., reduced insulin levels) only when administrated in low dosage, both via the bolus or via drinking water. However, under DEXA treatment, leucine administration was found to significantly influence this response, since leucine supplementation via drinking water clearly induced a diabetic state, whereas the same effect was not observed when supplied via the gavage.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.