Applications of Surface Plasmon Resonance (SPR) for the Characterization of Nanoparticles Developed for Biomedical Purposes

Sensors

Mara Canovi³  Jacopo Lucchetti³  Matteo Stravalaci³  Francesca Re²  Davide Moscatelli¹  Paolo Bigini³  Mario Salmona³ 

[1] Department of Chemistry, Materials and Chemical Engineering, Politecnico di Milano, Milano 20131, Italy; E-Mail:;Department of Experimental Medicine, University of Milano Bicocca, Monza 20052, Italy; E-Mail:;Department of Biochemistry and Molecular Pharmacology, Mario Negri Institute for Pharmacological Research, Milano 20156, Italy; E-Mails:

关键词: nanoparticles;    surface plasmon resonance;    protein corona;   

DOI  :  10.3390/s121216420

来源: mdpi

【 摘 要 】

Great interest is currently being devoted to the development of nanoparticles (NPs) for biomedical purposes, designed to improve the pharmacokinetic profile of their cargos (either imaging probes or drugs) and to enhance the specific targeting at the disease site. Recent works suggest that Surface Plasmon Resonance (SPR), widely used for the analysis of biomolecular interactions, represents a technique of choice for rapid and quantitative analyses of the interaction between NPs—functionalized with specific ligands—and their putative biological targets. Moreover, SPR can provide important details on the formation and the role of the protein “corona”, i.e., the protein layer which coats NPs once they come into contact with biological fluids. These novel applications of SPR sensors may be very useful to characterize, screen and develop nanodevices for biomedical purposes.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland