Methods, Challenges and Potentials of Single Cell RNA-seq

Biology

关键词: RNA-seq;    scRNA-seq;    single cell;   

DOI  :  10.3390/biology1030658

来源: mdpi

【 摘 要 】

RNA-sequencing (RNA-seq) has become the tool of choice for transcriptomics. Several recent studies demonstrate its successful adaption to single cell analysis. This allows new biological insights into cell differentiation, cell-to-cell variation and gene regulation, and how these aspects depend on each other. Here, I review the current single cell RNA-seq (scRNA-seq) efforts and discuss experimental protocols, challenges and potentials.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.