Mathematic Modeling for Optimum Conditions on Aflatoxin B1 Degradation by the Aerobic Bacterium Rhodococcus erythropolis

Toxins

Qing Kong¹  Cuiping Zhai¹  Bin Guan¹  Chunjuan Li²  Shihua Shan² 

[1] School of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China;Shandong Peanut Research Institute, Qingdao, Shandong 266100, China;

关键词: Rhodococcus erythropolis;    degradation efficiency;    optimization;    Plackett–Burman design;    central composite design;    response surface methodology;    aflatoxins;   

DOI  :  10.3390/toxins4111181

来源: mdpi

【 摘 要 】

Response surface methodology was employed to optimize the degradation conditions of AFB₁ by Rhodococcus erythropolis in liquid culture. The most important factors that influence the degradation, as identified by a two-level Plackett-Burman design with six variables, were temperature, pH, liquid volume, inoculum size, agitation speed and incubation time. Central composite design (CCD) and response surface analysis were used to further investigate the interactions between these variables and to optimize the degradation efficiency of R. erythropolis based on a second-order model. The results demonstrated that the optimal parameters were: temperature, 23.2 °C; pH, 7.17; liquid volume, 24.6 mL in 100-mL flask; inoculum size, 10%; agitation speed, 180 rpm; and incubation time, 81.9 h. Under these conditions, the degradation efficiency of R. erythropolis could reach 95.8% in liquid culture, which was increased by about three times as compared to non-optimized conditions. The result by mathematic modeling has great potential for aflatoxin removal in industrial fermentation such as in food processing and ethanol production.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.