Development of New Polymorphic Microsatellite Loci for the Barley Stem Gall Midge, Mayetiola hordei (Diptera: Cecidomyiidae) from an Enriched Library

International Journal of Molecular Sciences

Maha Mezghani-Khemakhem²  Dhia Bouktila²  Nathalie Casse¹  Houcine Maaroufi²  Mohamed Makni² 

[1] Laboratory Mer, Molécules, Santé (MMS), UFR Sciences and Techniques Le Mans, University of Maine, Le Mans 72085, France; E-Mail:;UR11ES10 Genomics of Crop Insect Pests, Faculté des Sciences de Tunis, University of Tunis El-Manar, El Manar 2092, Tunisia; E-Mails:

关键词: Mayetiola destructor;    dinucleotide;    trinucleotide;    molecular ecology;   

DOI  :  10.3390/ijms131114446

来源: mdpi

【 摘 要 】

Using an enriched library method, seven polymorphic microsatellite loci were isolated from the barley stem gall midge, Mayetiola hordei. Polymorphism at loci was surveyed on 57 individual midges collected on barley in Tunisia. Across loci, polymorphism ranged from two to six alleles per locus. The observed heterozygosity varied between 0.070 and 0.877. Based on the number of alleles detected and the associated levels of heterozygosity, we believe that these loci will prove useful for population genetic studies on M. hordei.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.