Integrated Amplification Microarrays for Infectious Disease Diagnostics

Microarrays

Darrell P. Chandler²  Lexi Bryant²  Sara B. Griesemer¹  Rui Gu¹  Christopher Knickerbocker²  Alexander Kukhtin²  Jennifer Parker²  Cynthia Zimmerman²  Kirsten St. George¹ 

[1] Laboratory of Viral Diseases, Wadsworth Center, New York State Dept of Health, 120 New Scotland Avenue, Albany, NY 12208, USA;Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick, MD 21701, USA;

关键词: microfluidics;    diagnostics;    gel element arrays;    asymmetric PCR;    RT-PCR;    reverse transcriptase;    multiplex;    integrated microarrays;   

DOI  :  10.3390/microarrays1030107

来源: mdpi

【 摘 要 】

This overview describes microarray-based tests that combine solution-phase amplification chemistry and microarray hybridization within a single microfluidic chamber. The integrated biochemical approach improves microarray workflow for diagnostic applications by reducing the number of steps and minimizing the potential for sample or amplicon cross-contamination. Examples described herein illustrate a basic, integrated approach for DNA and RNA genomes, and a simple consumable architecture for incorporating wash steps while retaining an entirely closed system. It is anticipated that integrated microarray biochemistry will provide an opportunity to significantly reduce the complexity and cost of microarray consumables, equipment, and workflow, which in turn will enable a broader spectrum of users to exploit the intrinsic multiplexing power of microarrays for infectious disease diagnostics.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.