Microarrays | |
Integrated Amplification Microarrays for Infectious Disease Diagnostics | |
Darrell P. Chandler2  Lexi Bryant2  Sara B. Griesemer1  Rui Gu1  Christopher Knickerbocker2  Alexander Kukhtin2  Jennifer Parker2  Cynthia Zimmerman2  Kirsten St. George1  | |
[1] Laboratory of Viral Diseases, Wadsworth Center, New York State Dept of Health, 120 New Scotland Avenue, Albany, NY 12208, USA;Akonni Biosystems, Inc., 400 Sagner Avenue, Suite 300, Frederick, MD 21701, USA; | |
关键词: microfluidics; diagnostics; gel element arrays; asymmetric PCR; RT-PCR; reverse transcriptase; multiplex; integrated microarrays; | |
DOI : 10.3390/microarrays1030107 | |
来源: mdpi | |
【 摘 要 】
This overview describes microarray-based tests that combine solution-phase amplification chemistry and microarray hybridization within a single microfluidic chamber. The integrated biochemical approach improves microarray workflow for diagnostic applications by reducing the number of steps and minimizing the potential for sample or amplicon cross-contamination. Examples described herein illustrate a basic, integrated approach for DNA and RNA genomes, and a simple consumable architecture for incorporating wash steps while retaining an entirely closed system. It is anticipated that integrated microarray biochemistry will provide an opportunity to significantly reduce the complexity and cost of microarray consumables, equipment, and workflow, which in turn will enable a broader spectrum of users to exploit the intrinsic multiplexing power of microarrays for infectious disease diagnostics.
【 授权许可】
CC BY
© 2012 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202003190040484ZK.pdf | 457KB | download |