Induction of Apoptosis, G0/G1 Phase Arrest and Microtubule Disassembly in K562 Leukemia Cells by Mere15, a Novel Polypeptide from Meretrix meretrix Linnaeus

Marine Drugs

Ming Liu²  Xiangzhong Zhao¹  Jin Zhao²  Lin Xiao²  Haizhou Liu²  Cuicui Wang²  Linyou Cheng²  Ning Wu² 

[1] Key Laboratory for Rare Diseases of Shandong Province, Institute of Basic Medicine, Shandong Academy of Medical Science, Jinan 250062, China;Institute of Oceanology, Chinese Academy of Science, 7 Nanhai Rd, Qingdao 266071, China;

关键词: Meretrix meretrix Linnaeus;    Mere15;    apoptosis;    anticancer;   

DOI  :  10.3390/md10112596

来源: mdpi

【 摘 要 】

Mere15 is a novel polypeptide from Meretrix meretrix Linnaeus with cytotoxicity in solid cancer cells. In this study, we investigated its activity on human K562 chronic myelogenous leukemia cells. Mere15 inhibited the growth of K562 cells with IC₅₀ values of 38.2 μg/mL. Mere15 also caused concentration dependent induction of apoptosis, with overproduction of reactive oxygen species and loss of mitochondrial membrane potential. Moreover, Mere15 arrested cell cycle progression at G₀/G₁ phase of K562 cells in a concentration dependent manner. In addition, Mere15 caused the disassembly of the microtubule cytoskeleton in K562 cells and inhibited the polymerization of tubulin in a cell free system via interaction with tubulin. We concluded that Mere15 was cytotoxic to K562 leukemia cells and the cytotoxicity was related to the apoptosis induction, cell cycle arrest and microtubule disassembly. These results implied that Merer15 was a broad spectrum anticancer polypeptide, not only cytotoxic to various solid cancer cells but also to the chronic myelogenous leukemia cells. Mere15 may have therapeutic potential for the treatment of leukemia.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.