International Journal of Molecular Sciences | |
Ginsenoside Rh2 Induces Human Hepatoma Cell Apoptosis via Bax/Bak Triggered Cytochrome C Release and Caspase-9/Caspase-8 Activation | |
Xiao-Xi Guo1  Qiao Guo1  Yang Li1  Seung Ki Lee2  Xue-Ning Wei1  | |
[1] Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, College of Life Science, Jilin University, Changchun 130012, China; E-Mails:;College of Pharmacy, Seoul National University, Seoul 151-742, Korea; E-Mail: | |
关键词: apoptosis; Bax/Bak translocation; caspase activation cascade; caspase-8/Bid pathway; ginsenoside Rh2; | |
DOI : 10.3390/ijms131215523 | |
来源: mdpi | |
【 摘 要 】
Ginsenoside Rh2 (G-Rh2) has been shown to induce apoptotic cell death in a variety of cancer cells. However, the details of the signal transduction cascade involved in G-Rh2-induced cell death is unclear. In this manuscript we elucidate the molecular mechanism of G-Rh2-induced apoptosis in human hepatoma SK-HEP-1 cells by demonstrating that G-Rh2 causes rapid and dramatic translocation of both Bak and Bax, which subsequently triggers mitochondrial cytochrome c release and consequent caspase activation. Interestingly, siRNA-based gene inactivation of caspase-8 effectively delays caspase-9 activation and apoptosis induced by G-Rh2, indicating that caspase-8 also plays an important role in the G-Rh2-induced apoptosis program. Taken together, our results indicate that G-Rh2 employs a multi pro-apoptotic pathway to execute cancer cell death, suggesting a potential role for G-Rh2 as a powerful chemotherapeutic agent.
【 授权许可】
CC BY
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.
【 预 览 】
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RO202003190040263ZK.pdf | 2012KB | download |