In Vitro Antimicrobial, Antioxidant, Cytotoxicity and GC-MS Analysis of Mazus goodenifolius

Molecules

Muhammad Riaz¹  Nasir Rasool¹  Iftikhar Hussain Bukhari¹  Muhammad Shahid¹  Muhammad Zubair¹  Komal Rizwan¹ 

[1] ¹Department of Chemistry, Government College University Faisalabad-38000, Pakistan ²Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad-38040, Pakistan ³Institute of Advanced Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia

关键词: Mazus goodenifolius;    antimicrobial;    antioxidant;    cytotoxicity;    GC-MS;   

DOI  :  10.3390/molecules171214275

来源: mdpi

【 摘 要 】

The antimicrobial, antioxidant and cytotoxic properties of Mazus goodenifolius (Hornem.) Pennell essential oil, methanol extract and some solvent-extracted subfractions of the latter were appraised. A qualitative, quantitative analysis of the classes of phytochemicals in the various fractions and GC-MS analysis of the essential oil was carried out. The activity of the plant extract and various subfractions against selected bacterial (Pasturella multocida, Escherichia coli, Bacillus subtilis and Staphylococcus aureus) and fungal strains (Aspergillus niger, Aspergillus flavus, Alternaria alternata and Rhizopus solani) was evaluated. The antioxidant activity was assayed using the DPPH radical scavenging and % inhibition of linoleic acid peroxidation tests. In the DPPH radical scavenging test the IC₅₀ values ranged from 7.21 to 91.79 µg/mL, and in the latter the range of % peroxidation inhibition was 35.42–93.48%. Protective effects of the absolute methanol extract, which had the highest content of phenolics and flavonoids, against H₂O₂ induced oxidative damage in plasmid pBR322 DNA was also evaluated, and it was found to offer some protection at the highest tested dose (1,000 µg/mL). Finally the cytotoxicity of the plant extract, fractions and essential oil was analyzed by examining haemolytic activity against human blood erythrocytes (RBCs), whereby the % lysis of RBCs was found to be in the range of 1.65 to 4.01%.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.