Antimicrobial and Cytotoxic Isohexenylnaphthazarins from Arnebia euchroma (Royle) Jonst. (Boraginaceae) Callus and Cell Suspension Culture

Molecules

Harilaos Damianakos¹  Nadine Kretschmer¹  Katarzyna Sykłowska-Baranek¹  Agnieszka Pietrosiuk¹  Rudolf Bauer¹ 

[1] ¹Department of Pharmacy, Institute of Pharmacognosy & Chemistry of Natural Products, University of Athens, Zografou 15771, Athens, Greece

关键词: Arnebia euchroma;    Boraginaceae;    callus culture;    cell suspension culture;    isohexenylnaphthazarins;    cytotoxic;    antimicrobial;   

DOI  :  10.3390/molecules171214310

来源: mdpi

【 摘 要 】

The phytochemical investigation of the n-hexane extract from callus and cell suspension culture of Arnebia euchroma (Royle) Jonst. resulted in the isolation of nine isohexenylnaphthazarins: deoxyalkannin (1), alkannin (2), acetylalkannin (3), isobutyrylalkannin (4), β-hydroxyisovalerylalkannin (5), 2''-(S)-α-methylbutyrylalkannin (6), propionylalkannin (7), teracrylalkannin (8) and acetylshikonin (9). Their structures were determined by MS and NMR spectroscopy. Pigments 2–8 are isolated for the first time from Arnebia in vitro cultures, 4 and 7 are reported in the present work as novel metabolites within the Arnebia genus, while 9 is a known constituent of both natural roots and in vitro cultures of A. euchroma. Moreover, methyl jasmonate and 1-monoglyceryl olate, palmitate and stearate are reported for the first time within the Boraginaceae family. The antimicrobial and cytotoxic activities of all isolated pigment compounds were tested, revealing a very interesting profile.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.