Sensors | |
Enhancement of Probe Signal for Screening of HIV-1 Protease Inhibitors in Living Cells | |
Huantong Yao1  | |
[1] Department of Biomedical Engineering, College of Engineering, 4188 Bell Engineering, University of Arkansas, Fayetteville, AR 72701, USA; E-Mail | |
关键词: molecular probe; FRET; HIV-1 protease inhibition; AcGFP1; mCherry; FLIM; high-content screening; | |
DOI : 10.3390/s121216759 | |
来源: mdpi | |
【 摘 要 】
The global human immunodeficiency virus infection/acquired immuno-deficiency syndrome (HIV/AIDS) epidemic is one of the biggest threats to human life. Mutation of the virus and toxicity of the existing drugs necessitate the development of new drugs for effective AIDS treatment. Previously, we developed a molecular probe that utilizes the Förster resonance energy transfer (FRET) principle to visualize HIV-1 protease inhibition within living cells for drug screening. We explored using AcGFP1 (a fluorescent mutant of the wild-type green fluorescent protein) as a donor and mCherry (a mutant of red fluorescent protein) as an acceptor for FRET microscopy imaging measurement of HIV-1 protease activity within living cells and demonstrated that the molecular probe is suitable for the High-Content Screening (HCS) of anti-HIV drugs through an automated FRET microscopy imaging measurement. In this study, we genetically engineered a probe with a tandem acceptor protein structure to enhance the probe’s signal. Both
【 授权许可】
CC BY
© 2012 by the authors; licensee MDPI, Basel, Switzerland
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