Isolation and Characterization of Eleven Polymorphic Microsatellite Loci for the Valuable Medicinal Plant Dendrobium huoshanense and Cross-Species Amplification

International Journal of Molecular Sciences

Hui Wang¹  Nai-Fu Chen²  Ji-Yang Zheng¹  Wen-Cai Wang¹  Yun-Yun Pei¹ 

[1] Key Laboratory of Molecular Evolution and Biodiversity and Anhui Provincial Key Laboratory of the Conservation and Exploitation of Biological Resources, College of Life Science, Anhui Normal University, Wuhu 241000, China; E-Mails:;Engineering Technology Research Center of Plant Cell Engineering of Anhui Province, Lu’an 237012, China; E-Mail:

关键词: Dendrobium huoshanense;    medicinal plant;    microsatellite;    molecular marker;    cross-amplification;    population genetics;   

DOI  :  10.3390/ijms131216779

来源: mdpi

【 摘 要 】

Dendrobium huoshanense (Orchidaceae) is a perennial herb and a widely used medicinal plant in Traditional Chinese medicine (TCM) endemic to Huoshan County town in Anhui province in Southeast China. A microsatellite-enriched genomic DNA library of D. huoshanense was developed and screened to identify marker loci. Eleven polymorphic loci were isolated and analyzed by screening 25 individuals collected from a natural population. The number of alleles per locus ranged from 2 to 5. The observed and expected heterozygosities ranged from 0.227 to 0.818 and from 0.317 to 0.757, respectively. Two loci showed significant deviations from Hardy-Weinberg equilibrium and four of the pairwise comparisons of loci revealed linkage disequilibrium (p < 0.05). These microsatellite loci were cross-amplified for five congeneric species and seven loci can be amplified in all species. These simple sequence repeats (SSR) markers are useful in genetic studies of D. huoshanense and other related species and in conservation decision-making.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.