An Amperometric Immunosensor Based on Multi-Walled Carbon Nanotubes-Thionine-Chitosan Nanocomposite Film for Chlorpyrifos Detection

Sensors

Xia Sun²  Yaoyao Cao²  Zhili Gong²  Xiangyou Wang¹  Yan Zhang² 

[1] School of Agriculture and Food Engineering, Shandong University of Technology, No.12, Zhangzhou Road, Zibo 255049, China;

关键词: multi-walled carbon nanotubes;    thionine;    chitosan;    chlorpyrifos;    immunosensor;   

DOI  :  10.3390/s121217247

来源: mdpi

【 摘 要 】

In this work, a novel amperometric immunosensor based on multi-walled carbon nanotubes-thionine-chitosan (MWCNTs-THI-CHIT) nanocomposite film as electrode modified material was developed for the detection of chlorpyrifos residues. The nanocomposite film was dropped onto a glassy carbon electrode (GCE), and then the anti-chlorpyrifos monoclonal antibody was covalently immobilized onto the surface of MWCNTs-THI-CHIT/GCE using the crosslinking agent glutaraldehyde (GA). The modification procedure was characterized by using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under the optimized conditions, a linear relationship between the relative change in peak current of different pulse voltammetry (DPV) and the logarithm of chlorpyrifos solution concentration was obtained in the range from 0.1 to 1.0 × 10⁵ ng/mL with a detection limit of 0.046 ng/mL. The proposed chlorpyrifos immunosensor exhibited high reproducibility, stability, and good selectivity and regeneration, making it a potential alternative tool for ultrasensitive detection of chlorpyrifos residues in vegetables and fruits.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland