An Ethanol Extract Derived from Bonnemaisoniahamifera Scavenges Ultraviolet B (UVB) Radiation-Induced Reactive Oxygen Species and Attenuates UVB-Induced Cell Damage in Human Keratinocytes

Marine Drugs

Mei Jing Piao³  Yu Jae Hyun³  Suk Ju Cho³  Hee Kyoung Kang³  Eun Sook Yoo³  Young Sang Koh³  Nam Ho Lee¹  Mi Hee Ko² 

[1] Department of Chemistry, College of Natural Sciences, Jeju National University, Jeju 690-756, Korea; E-Mail:;Jeju Biodiversity Research Institute, Jeju Technopark, Jeju 699-943, Korea; E-Mail:;School of Medicine, Jeju National University, Jeju 690-756, Korea; E-Mails:

关键词: Bonnemaisonia hamifera;    human keratinocytes;    photoprotection;    reactive oxygen species;    ultraviolet B;   

DOI  :  10.3390/md10122826

来源: mdpi

【 摘 要 】

The present study investigated the photoprotective properties of an ethanol extract derived from the red alga Bonnemaisonia hamifera against ultraviolet B (UVB)-induced cell damage in human HaCaT keratinocytes. The Bonnemaisoniahamifera ethanol extract (BHE) scavenged the superoxide anion generated by the xanthine/xanthine oxidase system and the hydroxyl radical generated by the Fenton reaction (FeSO₄ + H₂O₂), both of which were detected by using electron spin resonance spectrometry. In addition, BHE exhibited scavenging activity against the 1,1-diphenyl-2-picrylhydrazyl radical and intracellular reactive oxygen species (ROS) that were induced by either hydrogen peroxide or UVB radiation. BHE reduced UVB-induced apoptosis, as shown by decreased apoptotic body formation and DNA fragmentation. BHE also attenuated DNA damage and the elevated levels of 8-isoprostane and protein carbonyls resulting from UVB-mediated oxidative stress. Furthermore, BHE absorbed electromagnetic radiation in the UVB range (280–320 nm). These results suggest that BHE protects human HaCaT keratinocytes against UVB-induced oxidative damage by scavenging ROS and absorbing UVB photons, thereby reducing injury to cellular components.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.