First Report of Cowpea Mild Mottle Carlavirus on Yardlong Bean (Vigna unguiculata subsp. sesquipedalis) in Venezuela

Viruses

Miriam Brito²  Thaly Fernández-Rodríguez¹  Mario José Garrido²  Alexander Mej໚s¹  Mirtha Romano¹ 

[1] Biotechnology and Plant Virology Laboratory, Center for Microbiology and Cell Biology, Venezuelan Institute for Scientific Research (IVIC), ZIP 20632, Caracas 1020-A, Venezuela; E-Mails:;Vegetal Virology and Phytopathogenic Bacteria Laboratory, Institute of Agricultural Botany, Agronomy Faculty, Central University of Venezuela, ZIP 4579, Maracay 2101-A, Venezuela; E-Mails:

关键词: Vigna unguiculata;    sesquipedalis;    yardlong bean;    Cowpea mild mottle carlavirus;    CPMMV;    diagnosis;    RT-PCR;    Venezuela;   

DOI  :  10.3390/v4123804

来源: mdpi

【 摘 要 】

Yardlong bean (Vigna unguiculata subsp. sesquipedalis) plants with virus-like systemic mottling and leaf distortion were observed in both experimental and commercial fields in Aragua State, Venezuela. Symptomatic leaves were shown to contain carlavirus-like particles. RT-PCR analysis with carlavirus-specific primers was positive in all tested samples. Nucleotide sequences of the obtained amplicons showed 84%–74% similarity to corresponding sequences of Cowpea mild mottle virus (CPMMV) isolates deposited in the GenBank database. This is the first report of CPMMV in Venezuela and is thought to be the first report of CPMMV infecting yardlong bean.

【 授权许可】

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.