Horizontal Bilayer for Electrical and Optical Recordings

Materials

Philipp Bartsch¹  Claudius Walter¹  Philipp Selenschik¹  Alf Honigmann¹ 

[1] Biophysics, Department of Biology/Chemistry, University Osnabrueck, Barbarastr. 13, Osnabrueck 49076, Germany; E-Mails:

关键词: horizontal bilayer;    simultaneous electrical-optical recordings;    membrane dynamics;    lipid diffusion;    supported bilayer;   

DOI  :  10.3390/ma5122705

来源: mdpi

【 摘 要 】

Artificial bilayer containing reconstituted ion channels, transporters and pumps serve as a well-defined model system for electrophysiological investigations of membrane protein structure–function relationship. Appropriately constructed microchips containing horizontally oriented bilayers with easy solution access to both sides provide, in addition, the possibility to investigate these model bilayer membranes and the membrane proteins therein with high resolution fluorescence techniques up to the single-molecule level. Here, we describe a bilayer microchip system in which long-term stable horizontal free-standing and hydrogel-supported bilayers can be formed and demonstrate its prospects particularly for single-molecule fluorescence spectroscopy and high resolution fluorescence microscopy in probing the physicochemical properties like phase behavior of the bilayer-forming lipids, as well as in functional studies of membrane proteins.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.