Generation of a Monoclonal Antibody Specifically Reacting with Neuron-specific TATA-Box Binding Protein-Associated Factor 1 (N-TAF1)

Antibodies

Satoshi Makino²  Chiaki Masuda²  Satoshi Ando³  Gen Tamiya¹ 

[1] Advanced Molecular Epidemiology Research Institute, Faculty of Medicine, Yamagata University, Yamagata, 990-9585, Japan; E-Mail:;Molecular Neuroscience Research Center, Shiga University of Medical Science, Otsu, 520-2192, Japan; E-Mail:;Department of Molecular Life Science, Tokai University School of Medicine, Kanagawa, 259-1193, Japan; E-Mail:

关键词: TAF1;    transcription factor;    immunohistochemistry;    immunoprecipitation;   

DOI  :  10.3390/antib2010001

来源: mdpi

【 摘 要 】

TATA-box binding protein-associated factor 1 (TAF1), the largest subunit of the transcription factor IID complex, plays an important role in the RNA polymerase II-mediated gene transcription pathway regulating the transcription of a large number of genes related to cell division. The neuron-specific isoform of the TAF1 gene (N-TAF1) may have an essential role in neurons through transcriptional regulation of many neuron-specific genes. The present study reports the preparation and properties of a monoclonal antibody directed against N-TAF1. The monoclonal antibody, 3A-11F, specifically recognized N-TAF1 protein with no reactivity to TAF1 protein, as evidenced by immunocytochemistry and immunoprecipitation using cultured cells expressing recombinant N-TAF1 or TAF1 protein. Immunohistochemistry using 3A-11F showed that N-TAF1-imunoreactivity was detected in the nuclear region of neurons in the rat brain. The 3A-11F monoclonal antibody promises to be a useful tool for determining the expression pattern and biological function of N-TAF1 in the brain.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.