pH Dependence of the Fluorescence Lifetime of FAD in Solution and in Cells

International Journal of Molecular Sciences

Md. Serajul Islam¹  Masato Honma¹  Takakazu Nakabayashi¹  Masataka Kinjo² 

[1] Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan; E-Mails:;Faculty of Advanced Life Science, Hokkaido University, Sapporo 001-0021, Japan; E-Mail:

关键词: FAD;    autofluorescence;    living cell;    fluorescence lifetime imaging;    intracellular pH;    fluorescence decay;   

DOI  :  10.3390/ijms14011952

来源: mdpi

【 摘 要 】

We have studied physiological parameters in a living cell using fluorescence lifetime imaging of endogenous chromophores. In this study, pH dependence of the fluorescence lifetime of flavin adenine dinucleotide (FAD), that is a significant cofactor exhibiting autofluorescence, has been investigated in buffer solution and in cells. The fluorescence lifetime of FAD remained unchanged with pH 5 to 9 in solution. However, the fluorescence lifetime in HeLa cells was found to decrease with increasing intracellular pH, suggesting that pH in a single cell can be estimated from the fluorescence lifetime imaging of FAD without adding exogenous fluorescent probes.

【 授权许可】

CC BY   
© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.