Fluorescent Ratiometric Indicators Based on Cu(II)-Induced Changes in Poly(NIPAM) Microparticle Volume

Sensors

John Osambo¹  W. Rudolf Seitz¹  Daniel P. Kennedy¹  Roy P. Planalp¹  Aaron M. Jones²  Randy K. Jackson² 

[1] Department of Chemistry, University of New Hampshire, Durham, NH 03824, USA; E-Mails:;Department of Chemistry, University of Connecticut, Storrs, CT 06269, USA; E-Mails:

关键词: fluorescence resonance energy transfer;    copper;    polyNIPAM;    microparticles;    ratiometric indicator;    metal complex;   

DOI  :  10.3390/s130101341

来源: mdpi

【 摘 要 】

Microparticles consisting of the thermal responsive polymer N-isopropyl acrylamide (polyNIPAM), a metal ion-binding ligand and a fluorophore pair that undergoes fluorescence resonance energy transfer (FRET) have been prepared and characterized. Upon the addition of Cu(II), the microparticles swell or contract depending on whether charge is introduced or neutralized on the polymer backbone. The variation in microparticle morphology is translated into changes in emission of each fluorophore in the FRET pair. By measuring the emission intensity ratio between the FRET pair upon Cu(II) addition, the concentration of metal ion in solution can be quantified. This ratiometric fluorescent indicator is the newest technique in an ongoing effort to use emission spectroscopy to monitor Cu(II) thermodynamic activity in environmental water samples.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.