Yeast α-Glucosidase Inhibitory Phenolic Compounds Isolated from Gynura medica Leaf

International Journal of Molecular Sciences

Chao Tan²  Qunxing Wang¹  Chunhua Luo²  Sai Chen²  Qianyuan Li² 

[1] The First College of Clinical Medical Science, China Three Gorges University, Yichang 443003, Hubei, China;

关键词: Gynura medica;    yeast α-glucosidase;    flavonols;    phenolic acids;   

DOI  :  10.3390/ijms14022551

来源: mdpi

【 摘 要 】

Gynura medica leaf extract contains significant amounts of flavonols and phenolic acids and exhibits powerful hypoglycemic activity against diabetic rats in vivo. However, the hypoglycemic active constituents that exist in the plant have not been fully elaborated. The purpose of this study is to isolate and elaborate the hypoglycemic activity compounds against inhibition the yeast α-glucosidase in vitro. Seven phenolic compounds including five flavonols and two phenolic acids were isolated from the leaf of G. medica. Their structures were identified by the extensive NMR and mass spectral analyses as: kaempferol (1), quercetin (2), kaempferol-3-O-β-D-glucopyranoside (3), kaempferol-3-O-rutinoside (4), rutin (5), chlorogenic acid (6) and 3,5-dicaffeoylquinic acid methyl ester (7). All of the compounds except 1 and 3 were isolated for the first time from G. medica. Compounds 1–7 were also assayed for their hypoglycemic activity against yeast α-glucosidase in vitro. All of the compounds except 1 and 6 showed good yeast α-glucosidase inhibitory activity with the IC₅₀ values of 1.67 mg/mL, 1.46 mg/mL, 0.38 mg/mL, 0.10 mg/mL and 0.53 mg/mL, respectively.

【 授权许可】

CC BY   
© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.