Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7'8-dihydro-2'-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker

International Journal of Molecular Sciences

Silvia Borrego⁶  Antonio Vazquez¹  Francisco Dasí⁷  Concha Cerdá⁶  Antonio Iradi³  Carmen Tormos²  Julia M. Sánchez²  Leticia Bagán²  Javier Boix⁴  Cristl Zaragoza¹  Jordi Camps⁵ 

[1] Department of General Surgery and Digestive Diseases, General University Hospital, Avda. Tres Cruces s/n, Valencia 46014, Spain; E-Mails:;Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Valencia, INCLIVA. Avda. Blasco Ibañez 15, Valencia 46010, Spain; E-Mails:;Department of Physiology, Faculty of Medicine, University of Valencia, Avda. Blasco Ibañez 15, Valencia 46010, Spain; E-Mail:;Department of Pathology, Faculty of Medicine, University of Valencia, Avda. Blasco Ibañez 15, Valencia 46010, Spain; E-Mail:;Bimedical Research Center, Sant Joan University Hospital, Health Institute of Research Pere Virgili, Rovira i Virigili University, Reus 43201, Spain; E-Mail:;Clinical Analysis Service, CDB-General University Hospital, Avda. Tres Cruces s/n., Valencia 46014, Spain; E-Mails:;Valencia Clinical Hospital Research Foundation (INCLIVA), Avda. Blasco Ibáñez 15, Valencia 46010, Spain; E-Mail:

关键词: 8-oxo-dG;    DNA repair enzymes;    gastric cancer;    oxidative stress;    tumor marker;   

DOI  :  10.3390/ijms14023467

来源: mdpi

【 摘 要 】

We characterized the oxidative stress (OS) status by the levels of reduced/oxidized glutathione (GSH/GSSG), malondialdehyde (MDA) and the mutagenic base 8-oxo-7′8-dihydro-2′-deoxyguanosine (8-oxo-dG) in human gastric carcinoma (HGC) samples and compared the results with normal tissue from the same patients. We also analyzed 8-oxo-dG in peripheral mononuclear cells (PMNC) and urine from healthy control subjects and in affected patients in the basal state and one, three, six, nine and twelve months after tumor resection. The levels of DNA repair enzyme mRNA expression (hOGG1, RAD51, MUYTH and MTH1) were determined in tumor specimens and compared with normal mucosa. Tumor specimens exhibited increased levels of MDA and 8-oxo-dG compared with normal gastric tissue. GSH levels were also increased, while GSSG levels remained stable. DNA repair enzyme mRNA expression was induced in the tumor tissues. Levels of 8-oxo-dG were significantly elevated in both urine and PMNC of gastric cancer patients compared with healthy controls. After gastrectomy, the levels of the damaged base in urine and PMNC decreased progressively to values close to those found in the healthy population. The high levels of 8-oxo-dG in urine may be related to the increased induction of DNA repair activity in tumor tissue, and the changes observed after tumor resection support its potential use as a tumor marker.

【 授权许可】

CC BY   
© 2013 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.