Cytotoxic Effect of Clerosterol Isolated from Codium fragile on A2058 Human Melanoma Cells

Marine Drugs

Areum Daseul Kim³  Youngki Lee³  Sang-Hyuck Kang²  Gi Young Kim²  Hye Sun Kim¹ 

[1] Cancer Research Institute, Seoul National University College of Medicine, Seoul 110-799, Korea; E-Mail:;Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Korea; E-Mails:;School of Medicine, Jeju National University, Jeju 690-756, Korea; E-Mails:

关键词: clerosterol;    melanoma cell;    apoptosis;    mitochondrial membrane potential;   

DOI  :  10.3390/md11020418

来源: mdpi

【 摘 要 】

The cytotoxic effects and mechanism of action of clerosterol, isolated from the marine alga Codium fragile, were investigated in A2058 human melanoma cells. Clerosterol inhibited the growth of A2058 cells with an IC₅₀ of 150 µM and induced apoptotic cell death, as evidenced by DNA fragmentation, an increase in the number of sub-G₁ hypodiploid cells and the presence of apoptotic bodies. Clerosterol treatment caused the loss of mitochondrial membrane potential. Alterations in the expression of apoptosis-associated proteins in response to clerosterol treatment included upregulation of Bax, downregulation of Bcl-2 and activation of caspases 3 and 9. The pan-caspase inhibitor treatment attenuated the expression of the active form of caspases and cell death induced by clerosterol. The present results show that clerosterol exerts its cytotoxic effect in A2058 human melanoma cells by caspases-dependent apoptosis.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.