Viruses | |
Low Copper and High Manganese Levels in Prion Protein Plaques | |
Christopher J. Johnson1  P.U.P.A. Gilbert2  Mike Abrecht3  Katherine L. Baldwin5  Robin E. Russell1  Joel A. Pedersen5  Judd M. Aiken4  | |
[1] USGS National Wildlife Health Center, 6006 Schroeder Road, Madison, WI 53711, USA; E-Mails:;Department of Physics, University of Wisconsin-Madison, 1150 University Avenue, Madison, WI 53706, USA; E-Mails:;Centre for Prions and Protein Folding Diseases, AFNS, University of Alberta, Edmonton, Alberta, T6G 2M8, Canada; E-Mail:;Program in Cellular & Molecular Biology, University of Wisconsin-Madison, 425-G Henry Mall Madison, WI 53706, USA; E-Mail: | |
关键词: transmissible spongiform encephalopathy; X-ray photoemission; copper; manganese; prion; brain; | |
DOI : 10.3390/v5020654 | |
来源: mdpi | |
【 摘 要 】
Accumulation of aggregates rich in an abnormally folded form of the prion protein characterize the neurodegeneration caused by transmissible spongiform encephalopathies (TSEs). The molecular triggers of plaque formation and neurodegeneration remain unknown, but analyses of TSE-infected brain homogenates and preparations enriched for abnormal prion protein suggest that reduced levels of copper and increased levels of manganese are associated with disease. The objectives of this study were to: (1) assess copper and manganese levels in healthy and TSE-infected Syrian hamster brain homogenates; (2) determine if the distribution of these metals can be mapped in TSE-infected brain tissue using X-ray photoelectron emission microscopy (X-PEEM) with synchrotron radiation; and (3) use X-PEEM to assess the relative amounts of copper and manganese in prion plaques
【 授权许可】
CC BY
© 2013 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
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