Characterization of Proanthocyanidins in Stems of Polygonum multiflorum Thunb as Strong Starch Hydrolase Inhibitors

Molecules

Hongyu Wang³  Lixia Song³  Shengbao Feng¹  Yuancai Liu²  Gang Zuo²  Fuli Lai²  Guangyuan He¹  Mingjie Chen¹ 

[1] The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, College of Life Science and Technology, Huazhong University of Science & Technology, Luoyu Road 1037, Wuhan 430074, Hubei, China;Jing Brand Company, 169 Daye Avenue, Daye 435100, Hubei, China;Food Science and Technology Program, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117543, Singapore

关键词: P. multiflorum;    proanthocyanidins;    α-amylase;    (epi)catechin;   

DOI  :  10.3390/molecules18022255

来源: mdpi

【 摘 要 】

Characterzation of polyphenolic compounds in the stems of P. multiflorum was conducted using HPLC, high resolution LC-MS and LC-MSⁿ. Proanthocyanidins in particular were isolated in 4.8% yield using solvent extraction followed by Sephadex LH-20 fractionation. HPLC analysis using a diol column revealed oligomers (from dimer to nonamer) as minor components, with (epi)catechin monomeric units predominating, and oligomers with higher degree of polymerization being dominant. Thiolysis treatment of the proanthocyanidins using mercaptoacetic acid produced thioether derivatives of (epi)catechin as the major product and a mean value of the degree of polymerization of 32.6 was estimated from the ratio of terminal and extension units of the (epi)catechin. The isolated proanthocyanidins were shown to strongly inhibit α-amylase with an acarbose equivalence (AE) value of 1,954.7 µmol AE/g and inhibit α-glucosidase with an AE value of 211.1 µmol AE/g.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.