The Identification and Characterization of Chitotriosidase Activity in Pancreatin from Porcine Pancreas

Molecules

Chia-Rui Shen³  Chao-Lin Liu¹  Hsiao-Ping Lee² 

[1]Department of Chemical Engineering and Graduate School of Biochemical Engineering, Ming Chi University of Technology, 84 Gung-Juan Road, Taishan, Taipei 24301, Taiwan

[2]Department of Environment and Biotechnology, Refining & Manufacturing Research Institute, CPC Corporation, 217 Min-Sheng S. Rd, Chiayi 60051, Taiwan

[3] E-Mail:

[4]Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 33302, Taiwan

[5] E-Mail:

关键词: chitin;    chitosan;    oligosaccharides;    pancreatin;    chitotriosidase;   

DOI  :  10.3390/molecules18032978

来源: mdpi

【 摘 要 】

The versatile oligosaccharide biopolymers, chitin and chitosan, are typically produced using enzymatic processes. However, these processes are usually costly because chitinases and chitosanases are available in limited quantities. Fortunately, a number of commercial enzymes can hydrolyze chitin and chitosan to produce long chain chitin or chitosan oligosaccharides. Here, a platform to screen for enzymes with chitinase and chitosanase activities using a single gel with glycol chitin or glycol chitosan as a substrate was applied. SDS-resistant chitinase and chitosanase activities were observed for pancreatin. Its chitotriosidase had an optimal hydrolysis pH of 4 in the substrate specificity assay. This activity was thermally unstable, but independent of 2-mercaptoethanol. This is the first time a chitotriosidase has been identified in the hog. This finding suggests that oligochitosaccharides can be mass-produced inexpensively using pancreatin.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.