9G DNAChip Technology: Self-Assembled Monolayer (SAM) of ssDNA for Ultra-Sensitive Detection of Biomarkers

International Journal of Molecular Sciences

Satish Balasaheb Nimse¹  Keum-Soo Song²  Junghoon Kim²  Danishmalik Rafiq Sayyed¹ 

[1] Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon 200-702, Korea; E-Mails:;Biometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon 200-161, Korea; E-Mails:

关键词: self-assembly;    SAM of ssDNA;    biomarker;    9G DNAChip;    hybridization;    C-reactive protein;   

DOI  :  10.3390/ijms14035723

来源: mdpi

【 摘 要 】

A 9G DNAChip obtained by allowing the formation of a self-assembled monolayer (SAM) of oligonucleotides appended with nine consecutive guanines on the chip surface has been applied in the detection of biomarkers. Using a 9G DNAChip, biomarker in the concentration range of 4 pg/mL to 40 fg/mL can be easily differentiated in the buffer matrix. Moreover, it is the first time that a biomarker with a concentration of 40 fg/mL has been detected in a mixture of proteins without use of any signal amplification technique.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.