Fluorescent Imaging for Assessment of the Effect of Combined Application of Electroporation and Rifampicin on HaCaT Cells as a New Therapeutic Approach for Psoriasis

Sensors

Biliana Nikolova¹  Anelia Kostadinova¹  Borislav Dimitrov¹  Zhivko Zhelev¹  Rumiana Bakalova²  Ichio Aoki²  Tsuneo Saga² 

[1] Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Acad.G. Bonchev Str., bl. 21, Sofia 1113, Bulgaria; E-Mails:;Molecular Imaging Center, National Institute of Radiological Sciences, 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan; E-Mails:

关键词: fluorescent imaging;    electroporation;    electrochemotherapy;    rifampicin;    psoriasis;   

DOI  :  10.3390/s130303625

来源: mdpi

【 摘 要 】

The study aimed to clarify the role of electric pulses in combination with chemotherapy on the viability of keratinocyte cell line HaCaT, in the context of its application as a new therapeutic approach for psoriasis. The data show that electroporation of HaCaT cells in combination with rifampicin induces cytoskeleton disruption and increases permeability of cell monolayer due to cell-cell junctions' interruption, visualized by fluorescent imaging of E-cadherin and actin integrity. This was accompanied with synergistic reduction of cell viability. The study proposes a new opportunity for more effective skin treatment than chemotherapy. The future application of this electrochemotherapeutic approach for combined local treatment of psoriasis may have serous benefits because of a high possibility to avoid side-effects of conventional chemotherapy.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.