Marine Drugs | |
Improved Detection of Domoic Acid Using Covalently Immobilised Antibody Fragments | |
Mar J. Hortig࿎la1  | |
[1] Microbiology, NUI Galway, University Road, Galway, Ireland; E-Mail: | |
关键词: domoic acid; scFv antibody fragment; covalent immobilisation; protein engineering; cysteine; | |
DOI : 10.3390/md11030881 | |
来源: mdpi | |
【 摘 要 】
Antibody molecules, and antibody fragments in particular, have enormous potential in the development of biosensors for marine monitoring. Conventional immobilisation approaches used in immunoassays typically yield unstable and mostly incorrectly oriented antibodies, however, resulting in reduced detection sensitivities for already low concentration analytes. The 2H12 anti-domoic acid scFv antibody fragment was engineered with cysteine-containing linkers of two different lengths, distal to the antigen binding pocket, for covalent and correctly oriented immobilisation of the scFvs on functionalised solid supports. The
【 授权许可】
CC BY
© 2013 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190037840ZK.pdf | 311KB | download |