Identification of Amplified Fragment Length Polymorphism (AFLP) Markers Tightly Associated with Drought Stress Gene in Male Sterile and Fertile Salvia miltiorrhiza Bunge

International Journal of Molecular Sciences

Yuejin Zhang¹  Lijun Guo¹  Zhiming Shu²  Yiyue Sun²  Yuanyuan Chen²  Zongsuo Liang² 

[1] Shaanxi Research Center of TCM Fingerprinting and NP Library, College of Life Sciences, Northwest A&F University, Yangling 712100, Shaanxi, China;

关键词: AFLP;    bulked segregant analysis;    drought stress;    male sterility;    near-isogenic lines;    Salvia miltiorrhiza;    water deficit;    water stress;   

DOI  :  10.3390/ijms14036518

来源: mdpi

【 摘 要 】

Consistent grain yield in drought environment has attracted wide attention due to global climate change. However, the important drought-related traits/genes in crops have been rarely reported. Many near-isogenic lines (NILs) of male sterile and fertile Salvia miltiorrhiza have been obtained in our previous work through testcross and backcross in continuous field experiments conducted in 2006–2009. Both segregating sterile and fertile populations were subjected to bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) with 384 and 170 primer combinations, respectively. One out of 14 AFLP markers (E9/M3₂₄₆) was identified in treated fertile population as tightly linked to the drought stress gene with a recombination frequency of 6.98% and at a distance of 7.02 cM. One of 15 other markers (E2/M5₃₅₇) was identified in a treated sterile population that is closely associated with the drought stress gene. It had a recombination frequency of 4.65% and at a distance of 4.66 cM. Interestingly, the E9/M3₂₄₆ fragment was found to be identical to another AFLP fragment E11/M4₂₀₈ that was tightly linked to the male sterile gene of S. miltiorrhiza with 95% identity and e-value 4 × 10⁻⁹³. Blastn analysis suggested that the drought stress gene sequence showed higher identity with nucleotides in Arabidopsis chromosome 1–5.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.