Microsatellite Markers of Willow Species and Characterization of 11 Polymorphic Microsatellites for Salix eriocephala (Salicaceae), a Potential Native Species for Biomass Production in Canada

Plants

Aurélien Lauron-Moreau¹  Frຝéric E. Pitre¹  Luc Brouillet¹ 

[1] Institut de recherche en biologie végétale, Université de Montréal, 4101 Sherbrooke Est, Montréal, QC H1X 2B2, Canada;

关键词: allele data;    microsatellite markers;    Salicaceae;    Salix eriocephala;    willow;   

DOI  :  10.3390/plants2020203

来源: mdpi

【 摘 要 】

Biomass produced from dedicated plantations constitutes a source of renewable energy and is expected to play an important role in several countries in the coming decades. The cultivation of woody crops such as willows therefore raises several environmental issues. In North America, several native willows are potentially interesting for biomass producers. Willow trees are diverse but few species used for environmental applications have been the object of molecular genetic studies. Based on the sequenced poplar genome, 24 microsatellite markers were assayed on five native North American willow species: Salix amygdaloides, S. discolor, S. eriocephala, S. interior and S. nigra. Polymorphic microsatellite markers were used to characterize the allele data on the shrub Salix eriocephala, a North American species with economic potential. Eleven markers amplified and confirmed the potential of this species. Analysis of samples from six populations in eastern Canada showed that all markers were variable as well as polymorphic in at least one population. The number of alleles per locus ranged from 1 to 9 (mean 2.95) and showed that these microsatellite markers can be used to assess genetic diversity of North American willow species.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.