Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide

Nanomaterials

Po-Jung Jimmy Huang¹ 

[1] Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada; E-Mail

关键词: graphene;    DNA;    adsorption;    fluorescence;    separation;    gel electrophoresis;   

DOI  :  10.3390/nano3020221

来源: mdpi

【 摘 要 】

Separation of short single- and double-stranded DNA typically requires gel electrophoresis followed by DNA extraction, which is a time consuming process. Graphene oxide adsorbs single-stranded DNA more quickly than double-stranded ones, allowing for selective removal of the former with a simple mixing and centrifugation operation. The effect of DNA length and salt on adsorption selectivity has been characterized and its application in DNA melting curve measurement has been demonstrated.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.