Two New Monoterpene Glycosides from Qing Shan Lu Shui Tea with Inhibitory Effects on Leukocyte-Type 12-Lipoxygenase Activity

Molecules

Hideyuki Ito³  Akemi Otsuki¹  Hitomi Mori³  Peng Li³  Mai Kinoshita¹  Yuki Kawakami¹  Hideaki Tsuji¹  Ding Zhi Fang² 

[1] Faculty of Health and Welfare Sciences, Okayama Prefectural University, 111 Kuboki, Soja, Okayama 719-1197, Japan; E-Mails:;Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, 17 Section 3, South Renmin Road, Chengdu 610041, China; E-Mail:;Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 1-1-1 Tsushima-naka, Kita-ku, Okayama 700-8530, Japan; E-Mails:

关键词: Qing Shan Lu Shui;    Ligustrum robustum;    liguroside;    monoterpene glycoside;    leukocyte-type 12-lipoxygenase;    Chinese tea;    catechin;   

DOI  :  10.3390/molecules18044257

来源: mdpi

【 摘 要 】

We evaluated the inhibitory effect of 12 Chinese teas on leukocyte-type 12-lipoxygenase (LOX) activity. Tea catechins such as epigallocatechin gallate have been known to exhibit leukocyte-type 12-LOX inhibition. Qing Shan Lu Shui, which contains lower catechin levels than the other tested teas, suppressed leukocyte-type 12-LOX activity. To characterize the bioactive components of Qing Shan Lu Shui, leukocyte-type 12-LOX inhibitory activity–guided fractionation of the aqueous ethanol extract of the tea was performed, resulting in the isolation of two new monoterpene glycosides: liguroside A (1) and B (2). The structures of compounds 1 and 2 were characterized as (2E,5E)-7-hydroperoxy-3,7-dimethyl-2,5-octadienyl-O-(α-l-rhamnopyranosyl)-(1″→3′)-(4′″-O-trans-p-coumaroyl)-β-d-glucopyranoside and (2E,5E)-7-hydroperoxy-3,7-dimethyl-2,5-octa-dienyl-O-(α-l-rhamnopyranosyl)-(1″→3′)-(4′″-O-cis-p-coumaroyl)-β-d-glucopyranoside, respectively, based on spectral and chemical evidence. Ligurosides A (1) and B (2) showed inhibitory effects on leukocyte-type 12-LOX activity, with IC₅₀ values of 1.7 and 0.7 μM, respectively.

【 授权许可】

CC BY   
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