Cholesterol Dependent Uptake and Interaction of Doxorubicin in MCF-7 Breast Cancer Cells

International Journal of Molecular Sciences

Petra Weber¹  Michael Wagner¹ 

[1] Institut für Angewandte Forschung, Hochschule Aalen, Anton-Huber Str. 21, 73430 Aalen, Germany;

关键词: cancer cells;    doxorubicin;    cholesterol;    fluorescence lifetime imaging microscopy (FLIM);   

DOI  :  10.3390/ijms14048358

来源: mdpi

【 摘 要 】

Methods of fluorescence spectroscopy and microscopy—including intensity and lifetime (FLIM) images—are used to examine uptake, intracellular location and interaction of the chemotherapeutic drug doxorubicin in MCF-7 human breast cancer cells as a function of cholesterol content. By comparing cells with natural and decreased cholesterol levels after 2 h or 24 h incubation with doxorubicin, we observed that higher fluorescence intensities and possibly shortened fluorescence lifetimes—reflecting increased uptake of the drug and more pronounced drug response—are concomitant with higher membrane fluidity.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland