Bio-Hydrogen Production from Pineapple Waste Extract by Anaerobic Mixed Cultures

Energies

Alissara Reungsang¹ 

[1] Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand

关键词: pineapple waste extract;    bio-hydrogen;   

DOI  :  10.3390/en6042175

来源: mdpi

【 摘 要 】

A statistical experimental design was employed to optimize factors that affect the production of hydrogen from the glucose contained in pineapple waste extract by anaerobic mixed cultures. Results from Plackett-Burman design indicated that substrate concentration, initial pH and FeSO₄ concentration had a statistically significant (p ≤ 0.05) influence on the hydrogen production potential (P_s) and the specific hydrogen production rate (SHPR). The path of steepest ascent was undertaken to approach the optimal region of these three significant factors which was then optimized using response surface methodology (RSM) with central composite design (CCD). The presence of a substrate concentration of 25.76 g-total sugar/L, initial pH of 5.56, and FeSO₄ concentration of 0.81 g/L gave a maximum predicted P_s of 5489 mL H₂/L, hydrogen yield of 1.83 mol H₂/mol glucose, and SHPR of 77.31 mL H₂/g-volatile suspended solid (VSS) h. A verification experiment indicated highly reproducible results with the observed P_s and SHPR being only 1.13% and 1.14% different from the predicted values.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.