Sensors | |
Performance Improvement of the One-Dot Lateral Flow Immunoassay for Aflatoxin B1 by Using a Smartphone-Based Reading System | |
Sangdae Lee2  Giyoung Kim1  | |
[1] Post-Harvest & Food Engineering Division, Department of Agricultural Engineering, National Academy of Agricultural Sciences, Suwon 441707, Korea; | |
关键词: lateral flow immunoassay; aflatoxin B1; Smartphone; point-of-care; | |
DOI : 10.3390/s130405109 | |
来源: mdpi | |
【 摘 要 】
This study was conducted to develop a simple, rapid, and accurate lateral flow immunoassay (LFIA) detection method for point-of-care diagnosis. The one-dot LFIA for aflatoxin B1 (AFB1) was based on the modified competitive binding format using competition between AFB1 and colloidal gold-AFB1-BSA conjugate for antibody binding sites in the test zone. A Smartphone-based reading system consisting of a Samsung Galaxy S2 Smartphone, a LFIA reader, and a Smartphone application for the image acquisition and data analysis. The detection limit of one-dot LFIA for AFB1 is 5 μg/kg. This method provided semi-quantitative analysis of AFB1 samples in the range of 5 to 1,000 μg/kg. Using combination of the one-dot LFIA and the Smartphone-based reading system, it is possible to conduct a more fast and accurate point-of-care diagnosis.
【 授权许可】
CC BY
© 2013 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190036928ZK.pdf | 543KB | download |