Unusual Glycosaminoglycans from a Deep Sea Hydrothermal Bacterium Improve Fibrillar Collagen Structuring and Fibroblast Activities in Engineered Connective Tissues

Marine Drugs

Karim Senni²  Farida Gueniche²  Sylvie Changotade³  Dominique Septier²  Corinne Sinquin¹  Jacqueline Ratiskol¹  Didier Lutomski³  Gaston Godeau²  Jean Guezennec¹ 

[1] Laboratory of Biotechnology and Marine Molecules, IFREMER, B.P. 21105 44311 Nantes, France; E-Mails:;Biochemistry Department, Dental School, Paris Descartes University, PRES Sorbonne Paris Cité, 1 rue Maurice Arnoux, Montrouge 92120, France; E-Mails:;UMR CNRS 7244, CSPBAT-LBPS, UFR SMBH, Paris 13 University, PRES Sorbonne Paris Cité, 74 Rue Marcel Cachin, Bobigny 93017, France; E-Mails:

关键词: marine hydrothermal bacteria;    glycosaminoglycan-mimetic;    collagen;    matrix metalloproteinases;    dermal fibroblast;    polysaccharides;    tissue engineering;   

DOI  :  10.3390/md11041351

来源: mdpi

【 摘 要 】

Biopolymers produced by marine organisms can offer useful tools for regenerative medicine. Particularly, HE800 exopolysaccharide (HE800 EPS) secreted by a deep-sea hydrothermal bacterium displays an interesting glycosaminoglycan-like feature resembling hyaluronan. Previous studies demonstrated its effectiveness to enhance in vivo bone regeneration and to support osteoblastic cell metabolism in culture. Thus, in order to assess the usefulness of this high-molecular weight polymer in tissue engineering and tissue repair, in vitro reconstructed connective tissues containing HE800 EPS were performed. We showed that this polysaccharide promotes both collagen structuring and extracellular matrix settle by dermal fibroblasts. Furthermore, from the native HE800 EPS, a low-molecular weight sulfated derivative (HE800 DROS) displaying chemical analogy with heparan-sulfate, was designed. Thus, it was demonstrated that HE800 DROS mimics some properties of heparan-sulfate, such as promotion of fibroblast proliferation and inhibition of matrix metalloproteinase (MMP) secretion. Therefore, we suggest that the HE800EPS family can be considered as an innovative biotechnological source of glycosaminoglycan-like compounds useful to design biomaterials and drugs for tissue engineering and repair.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.