IGF-1 Antibody Prolongs the Effective Duration Time of Botulinum Toxin in Decreasing Muscle Strength

International Journal of Molecular Sciences

Lingjing Jin²  Lizhen Pan²  Wuchao Liu²  Yan Guo²  Yuguo Zheng¹  Qiang Guan² 

[1] Department of Biomedicine, China National Center for Biotechnology Development, Beijing 100036, China; E-Mail:;Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China; E-Mails:

关键词: botulinum toxin;    insulin-like growth factor 1;    muscle strength;    muscle-specific receptor tyrosine kinase;   

DOI  :  10.3390/ijms14059051

来源: mdpi

【 摘 要 】

Botulinum toxin type-A (Btx-A), a powerful therapeutic tool in various medical specialties, requires repeated injections to maintain its effect. Therefore, novel methods to prolong the effective duration time of Btx-A are highly needed. Rats were assigned to three major groups: control group (n = 30), Btx-A group (n = 30), and IGF-1 Ab groups. IGF-1 Ab groups were composed by sub-groups A1–A5 (each has 25 rats) for the subsequent IGF-1Ab dose-effect study. Muscle strength was determined by a survey system for rat lower limbs nerve and muscle function. Muscle-specific receptor tyrosine kinase (MuSK), Insulin-like growth factor binding protein-5 (IGFBP5), and growth-associated protein, 43-kDa (GAP43) were determined by real-time polymerase chain reactions (PCRs) and Western blot. We found that Btx-A decreased the muscle strength, with a paralysis maintained for 70 days. IGF-1Ab prolonged the effective duration time of Btx-A. Real-time PCRs and Western blot showed that IGF-1Ab delayed the increase of MuSK and IGFBP5 after Btx-A injection, without affecting GAP43. These results indicate that IGF-1Ab might prolong the effective duration time of Btx-A on muscle strength through delaying the increase of MuSK. It would be interesting to determine whether IGF-1Ab can be used as an auxiliary measure to the Btx-A treatment in the future.

【 授权许可】

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