Dual-Specific Interaction to Detect DNA on Gold Nanoparticles

Sensors

Chuan-Liang Feng¹  Xiao-Qiu Dou¹  Qing-Lei Liu¹  Wang Zhang¹  Jia-Jun Gu¹  Shen-Min Zhu¹  Andrew Tobias Aveling Jenkins² 

[1] State Key Lab of Metal Matrix Composites, School of Materials Science & Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; E-Mails:;Department of Chemistry, University of Bath, Bath BA2 7AY, UK; E-Mail:

关键词: nanoparticles;    fluorophore dye;    quenching;    streptavidin/biotin;    DNA hybridization;    sensors;   

DOI  :  10.3390/s130505749

来源: mdpi

【 摘 要 】

An approach to selectively and efficiently detect single strand DNA is developed by using streptavidin coated gold nanoparticles (StAuNPs) as efficient quenchers. The central concept for the successful detection is the combination the of streptavidin-biotin interaction with specific probe-target DNA hybridization. Biotin labeled probe DNAs act as “bridges” to bring Cy5 labeled targets to the particle surface and the fluorophore dye can be rapidly and efficiently quenched by StAuPNs. By measuring the changes of photoluminescence intensity of Cy5, an efficient, selective, and reversed detection of DNA hybridization is realized. The methodology may pave a new way for simple and rapid detections of biomolecules.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.