International Journal of Molecular Sciences | |
Resistin Promotes the Expression of Vascular Endothelial Growth Factor in Ovary Carcinoma Cells | |
Li Pang2  Yi Zhang1  Yu Yu2  | |
[1] Department of Gynecology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China; E-Mail:;Department of Obstetrics and Gynecology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning, China; E-Mails: | |
关键词: resistin; vascular endothelial growth factor (VEGF); Sp1; phosphoinositide 3-kinase (PI3K)/Akt; | |
DOI : 10.3390/ijms14059751 | |
来源: mdpi | |
【 摘 要 】
Resistin is a novel hormone that is secreted by human adipocytes and mononuclear cells and is associated with obesity, insulin resistance and inflammation. Recently, resistin has been postulated to play a role in angiogenesis. Here, we investigated the hypothesis that resistin regulates ovary carcinoma production of vascular endothelial growth factor (VEGF) and the angiogenic processes. We found that in human ovarian epithelial carcinoma cells (HO-8910), resistin (10–150 ng/mL) enhanced both VEGF protein and mRNA expression in a time- and concentration-dependent manner, as well as promoter activity. Furthermore, resistin enhanced DNA-binding activity of Sp1 with VEGF promoter in a PI3K/Akt-dependent manner. PI3K/Akt activated by resistin led to increasing interaction with Sp1, triggering a progressive phosphorylation of Sp1 on Thr453 and Thr739, resulting in the upregulation of VEGF expression. In an
【 授权许可】
CC BY
© 2013 by the authors; licensee MDPI, Basel, Switzerland
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