Isolation of Nematicidal Triterpenoid Saponins from Pulsatilla koreana Root and Their Activities against Meloidogyne incognita

Molecules

Wei Li²  Ya Nan Sun²  Xi Tao Yan²  Seo Young Yang²  Suk Jun Lee¹  Hyo Jeung Byun¹  Chang Sup Moon¹  Byung Soo Han¹ 

[1] Technical Research Institute, Dongbang Agro Co., LTD, Buyeo, Chungnam 323-932, Korea;College of Pharmacy, Chungnam National University, Daejeon 305-764, Korea

关键词: Pulsatilla koreana;    triterpene saponin;    Meloidogyne incognita;    nematicidal activity;   

DOI  :  10.3390/molecules18055306

来源: mdpi

【 摘 要 】

Pulsatilla koreana, a species endemic to Korea, is an important herb used in traditional medicine to treat amoebic dysentery and malaria. In the present study, 23 oleanane-type triterpenoid saponins 1–23 and eight lupane-type triterpenoid saponins 24–31 were isolated from the roots of P. koreana. Their structures were elucidated on the basis of spectroscopic data. The methanol extract and isolated compounds were next assessed for nematicidal activity against the root-knot nematode (Meloidogyne incognita). The methanol extract showed strong nematicidal activity after 48 h, with a LC₅₀ value of 92.8 μg/mL. Compounds 2, 5, 9, 20, and 21 showed significant effects, with LC₅₀ values ranging from 70.1 to 94.7 μg/mL after 48 h. These results suggest that triterpenoid saponins from P. koreana should be explored as potential natural nematicides for developing new agents to control root-knot nematode disease.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.