Oxidative and Molecular Responses in Capsicum annuum L. after Hydrogen Peroxide, Salicylic Acid and Chitosan Foliar Applications

International Journal of Molecular Sciences

Laura Mej໚-Teniente²  Flor de Dalia Durán-Flores²  Angela Mar໚ Chapa-Oliver²  Irineo Torres-Pacheco²  Andrés Cruz-Hernández²  Mario M. González-Chavira¹  Rosal໚ V. Ocampo-Velázquez² 

[1] Biotechnology Group, National Institute for Forestry, Agriculture and Livestock Research (INIFAP), Celaya-San Miguel de Allende, km 6, CP 38010, Celaya, Guanajuato, Mexico; E-Mail:;Biosystems Engineering Group, School of Engineering, Queretaro Autonomous University, C.U Cerro de las Campanas, S/N, colonia Las Campanas, CP 76010, Santiago de Querétaro, Querétaro, Mexico; E-Mails:

关键词: reactive oxygen species;    oxidative stress;    elicitors;    oxidative stress dynamic;    hydrogen peroxide;    salicylic acid;    chitosan;    catalase;    phenylalanine ammonia lyase;   

DOI  :  10.3390/ijms140510178

来源: mdpi

【 摘 要 】

Hydrogen peroxide (H₂O₂) is an important ROS molecule (Reactive oxygen species) that serves as a signal of oxidative stress and activation of signaling cascades as a result of the early response of the plant to biotic stress. This response can also be generated with the application of elicitors, stable molecules that induce the activation of transduction cascades and hormonal pathways, which trigger induced resistance to environmental stress. In this work, we evaluated the endogenous H₂O₂ production caused by salicylic acid (SA), chitosan (QN), and H₂O₂ elicitors in Capsicum annuum L. Hydrogen peroxide production after elicitation, catalase (CAT) and phenylalanine ammonia lyase (PAL) activities, as well as gene expression analysis of cat1, pal, and pathogenesis-related protein 1 (pr1) were determined. Our results displayed that 6.7 and 10 mM SA concentrations, and, 14 and 18 mM H₂O₂ concentrations, induced an endogenous H₂O₂ and gene expression. QN treatments induced the same responses in lesser proportion than the other two elicitors. Endogenous H₂O₂ production monitored during several days, showed results that could be an indicator for determining application opportunity uses in agriculture for maintaining plant alert systems against a stress.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland