Development of Amperometric Biosensors Based on Nanostructured Tyrosinase-Conducting Polymer Composite Electrodes

Sensors

Stelian Lupu¹  Cecilia Lete³  Paul Cătălin Balaure²  Dan Ion Caval¹  Constantin Mihailciuc⁴  Boris Lakard⁵  Jean-Yves Hihn⁵ 

[1] Department of Analytical Chemistry and Environmental Engineering, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Gheorghe 1-5, Bucharest 011061, Romania; E-Mail:;Department of Organic Chemistry, Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu Gheorghe 1-5, Bucharest 011061, Romania; E-Mail:;Laboratory of Electrochemistry, Institute of Physical Chemistry “Ilie Murgulescu” of the Romanian Academy, Splaiul Independentei 202, Bucharest 060021, Romania; E-Mail:;Department of Physical Chemistry, Faculty of Chemistry, University of Bucharest, Bld. Regina Elisabeta 4-12, Bucharest 030018, Romania; E-Mail:;Institut UTINAM, CNRS-UMR 6213, Université de Franche-Comté, 16 route de Gray, Besançon Cedex 25030, France; E-Mails:

关键词: amperometric biosensor;    microelectrode arrays;    polythiophene enzyme biosensor;    tyrosinase-polythiophene composite;    alternating waveform polymerization potential;    dopamine;    catechol;   

DOI  :  10.3390/s130506759

来源: mdpi

【 摘 要 】

Bio-composite coatings consisting of poly(3,4-ethylenedioxythiophene) (PEDOT) and tyrosinase (Ty) were successfully electrodeposited on conventional size gold (Au) disk electrodes and microelectrode arrays using sinusoidal voltages. Electrochemical polymerization of the corresponding monomer was carried out in the presence of various Ty amounts in aqueous buffered solutions. The bio-composite coatings prepared using sinusoidal voltages and potentiostatic electrodeposition methods were compared in terms of morphology, electrochemical properties, and biocatalytic activity towards various analytes. The amperometric biosensors were tested in dopamine (DA) and catechol (CT) electroanalysis in aqueous buffered solutions. The analytical performance of the developed biosensors was investigated in terms of linear response range, detection limit, sensitivity, and repeatability. A semi-quantitative multi-analyte procedure for simultaneous determination of DA and CT was developed. The amperometric biosensor prepared using sinusoidal voltages showed much better analytical performance. The Au disk biosensor obtained by 50 mV alternating voltage amplitude displayed a linear response for DA concentrations ranging from 10 to 300 μM, with a detection limit of 4.18 μM.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.