Glyphosate-Resistant Goosegrass from Mississippi

Agronomy

William T. Molin¹  Alice A. Wright² 

[1] Crop Production Systems Research Unit, USDA-ARS, 141 Experiment Station Road, Stoneville, MS 38776, USA;

关键词: glyphosate resistance;    point mutation;    single nucleotide polymorphism;    Eleusine indica;   

DOI  :  10.3390/agronomy3020474

来源: mdpi

【 摘 要 】

A suspected glyphosate-resistant goosegrass [Eleusine indica (L.) Gaertn.] population, found in Washington County, Mississippi, was studied to determine the level of resistance and whether the resistance was due to a point mutation, as was previously identified in a Malaysian population. Whole plant dose response assays indicated a two- to four-fold increase in resistance to glyphosate. Leaf disc bioassays based on a glyphosate-dependent increase in shikimate levels indicated a five- to eight-fold increase in resistance. Sequence comparisons of messenger RNA for epsps, the gene encoding the enzyme 5-enolpyruvylshikimate-3-phosphate synthase, from resistant and sensitive goosegrass, revealed a cytosine to thymine nucleotide change at position 319 in the resistant accessions. This single nucleotide polymorphism causes a proline to serine amino acid substitution at position 106 in 5-enolpyruvylshikimate-3-phosphate synthase. A real-time polymerase chain reaction assay using DNA probes specific for the nucleotide change at position 319 was developed to detect this polymorphism. Goosegrass from 42 locations were screened, and the results indicated that glyphosate-resistant goosegrass remained localized to where it was discovered. Pendimethalin, s-metolachlor, clethodim, paraquat and fluazifop controlled resistant goosegrass 93% to 100%, indicating that several control options for glyphosate-resistant goosegrass are available.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.