Effect of Leguminous Lectins on the Growth of Rhizobium tropici CIAT899

Molecules

Mayron Alves de Vasconcelos⁴  Cláudio Oliveira Cunha⁴  Francisco Vassiliepe Sousa Arruda¹  Victor Alves Carneiro⁴  Rafaela Mesquita Bastos¹  Fปio Martins Mercante²  Kyria Santiago do Nascimento⁴  Benildo Sousa Cavada⁴  Ricardo Pires dos Santos³ 

[1] Integrated Laboratory of Biomolecules (LIBS), Department of Pathology and Legal Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, CE, 60430-160, Brazil; E-Mails:;Embrapa Western Region Agriculture, Dourados, MS, 79804-970, Brazil; E-Mail:;Laboratory of Materials Engineering and Computation of Sobral (LEMCS), Federal University of Ceará, Campus do Derby, Sobral, CE, 62042-280, Brazil; E-Mail:;Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza, CE, 60440-970, Brazil; E-Mails:

关键词: rhizobia;    Phaseolus vulgaris;    stimulus;    inoculant additive;   

DOI  :  10.3390/molecules18055792

来源: mdpi

【 摘 要 】

Rhizobium tropici is a Gram-negative bacterium that induces nodules and fixed atmospheric nitrogen in symbiotic association with Phaseolus vulgaris (common bean) and some other leguminous species. Lectins are proteins that specifically bind to carbohydrates and, consequently, modulate different biological functions. In this study, the d-glucose/d-mannose-binding lectins (from seeds of Dioclea megacarpa, D. rostrata and D. violacea) and d-galactose-binding lectins (from seeds of Bauhinia variegata, Erythina velutina and Vatairea macrocarpa) were purified using chromatographic techniques and evaluated for their effect on the growth of R. tropici CIAT899. All lectins were assayed with a satisfactory degree of purity according to SDS-PAGE analysis, and stimulated bacterial growth; in particular, the Dioclea rostrata lectin was the most active among all tested proteins. As confirmed in the present study, both d-galactose- and d-glucose/d-mannose-binding lectins purified from the seeds of leguminous plants may be powerful biotechnological tools to stimulate the growth of R. tropici CIAT99, thus improving symbiotic interaction between rhizobia and common bean and, hence, the production of this field crop.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.