The Interaction of CuS and Halothiobacillus HT1 Biofilm in Microscale Using Synchrotron Radiation-Based Techniques

International Journal of Molecular Sciences

Huirong Lin²  Guangcun Chen²  Shenhai Zhu²  Yingxu Chen²  Dongliang Chen³  Wei Xu³  Xiaohan Yu¹ 

[1] Institute of Applied Physics, Chinese Academy of Science, Shanghai Synchrotron Radiation Facility, Shanghai 201800, China; E-Mail:;Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China; E-Mails:;Institute of High Energy Physics, Chinese Academy of Science, Beijing Synchrotron Radiation Facility, Beijing 100049, China; E-Mails:

关键词: Halothiobacillus HT1 biofilm;    distribution;    speciation;    copper;    sulfur;   

DOI  :  10.3390/ijms140611113

来源: mdpi

【 摘 要 】

In order to investigate the microbe-mineral interaction in the micro scale, spatial distribution and speciation of Cu and S in Halothiobacillus HT1 biofilm formed on a CuS surface was examined using synchrotron-based X-ray techniques. Confocal laser scanning microscope (CLSM) results indicated that Halothiobacillus HT1 biofilm formation gave rise to distinct chemical and redox gradients, leading to diverse niches in the biofilm. Live cells were distributed at the air-biofilm and membrane-biofilm interface. CuS was oxidized by Halothiobacillus HT1 biofilm, and copper penetrated into the biofilm. Sulfide was oxidized to cysteine (77.3%), sulfite (3.8%) and sulfonate (18.9%). Cu-cysteine-like species were involved in the copper homeostasis. These results significantly improve our understanding of the interfacial properties of the biofilm-mineral interface.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland