A New Antiproliferative and Antioxidant Peptide Isolated from Arca subcrenata

Marine Drugs

Lili Chen³  Liyan Song¹  Tingfei Li²  Jianhua Zhu³  Jian Xu³  Qin Zheng¹ 

[1] College of Pharmacy, Jinan University, Guangzhou 510632, China; E-Mail:;Guangdong Food and Drug Vocational-Technical School, Guangzhou 510663, China; E-Mail:;Biotechnological Institute of Chinese Materia Medica, Jinan University, Guangzhou 510632, China; E-Mails:

关键词: Arca subcrenata;    peptide;    antiproliferative activity;    antioxidant activity;   

DOI  :  10.3390/md11061800

来源: mdpi

【 摘 要 】

A new antitumor and antioxidant peptide (H3) was isolated from Arca subcrenata Lischke using ion exchange and hydrophobic column chromatography. The purity of H3 was over 99.3% in reversed phase-high performance liquid chromatography (RP-HPLC) and the molecular weight was determined to be 20,491.0 Da by electrospray-ionization mass spectrometry (ESI-MS/MS). The isoelectric point of H3 was measured to be 6.65 by isoelectric focusing-polyacrylamide gel electrophoresis. Partial amino acid sequence of this peptide was determined as ISMEDVEESRKNGMHSIDVNHDGKHRAYWADNTYLM-KCMDLPYDVLDTGGKDRSSDKNTDLVDLFELDMVPDRKNNECMNMIMDVIDTN-TAARPYYCSLDVNHDGAGLSMEDVEEDK via MALDI-TOF/TOF-MS and de novo sequencing. The in vitro antitumor activity of H3 was evaluated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The result indicated that H3 exhibited significant antiproliferative activity against HeLa, HepG2 and HT-29 cell lines with IC₅₀ values of 10.8, 10.1 and 10.5 μg/mL. The scavenging percentage of H3 at 8 mg/mL to 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radicals were 56.8% and 47.5%, respectively.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.