Prevention of Intra-Abdominal Adhesion by Bi-Layer Electrospun Membrane

International Journal of Molecular Sciences

Shichao Jiang¹  Wei Wang¹  Hede Yan² 

[1] Department of Orthopaedics, Shanghai Sixth People′s Hospital, Shanghai Jiaotong University School of Medicine, 600 Yishan Road, Shanghai 200233, China;

关键词: postoperative adhesion;    hyaluronic acid;    electrospun;    barrier;    adhesion prevention;   

DOI  :  10.3390/ijms140611861

来源: mdpi

【 摘 要 】

The aim of this study was to compare the anti-adhesion efficacy of a bi-layer electrospun fibrous membrane consisting of hyaluronic acid-loaded poly(ɛ-caprolactone) (PCL) fibrous membrane as the inner layer and PCL fibrous membrane as the outer layer with a single-layer PCL electrospun fibrous membrane in a rat cecum abrasion model. The rat model utilized a cecal abrasion and abdominal wall insult surgical protocol. The bi-layer and PCL membranes were applied between the cecum and the abdominal wall, respectively. Control animals did not receive any treatment. After postoperative day 14, a visual semiquantitative grading scale was used to grade the extent of adhesion. Histological analysis was performed to reveal the features of adhesion tissues. Bi-layer membrane treated animals showed significantly lower adhesion scores than control animals (p < 0.05) and a lower adhesion score compared with the PCL membrane. Histological analysis of the bi-layer membrane treated rat rarely demonstrated tissue adhesion while that of the PCL membrane treated rat and control rat showed loose and dense adhesion tissues, respectively. Bi-layer membrane can efficiently prevent adhesion formation in abdominal cavity and showed a significantly decreased adhesion tissue formation compared with the control.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland